Learning objective
Required practical 7: use chromatography to investigate pigments isolated from leaves.
Read the explanation, check the common trap, then practise with flashcards and questions.
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Topic
Energy transfers official content
Subtopic
Photosynthesis
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Quick explanation
Required practical 7: use chromatography to investigate pigments isolated from leaves
- This point belongs to Energy transfers official content, especially Photosynthesis.
- You need to be able to required practical 7: use chromatography to investigate pigments isolated from leaves.
- The key ideas to know are required practical 7.
- Use the linked flashcards and practice questions to check recall, then practise applying the idea in an exam-style answer.
Key concepts
Why it matters
This objective helps connect Photosynthesis to exam-style questions, flashcards, and revision notes for Energy transfers official content.
Quick student answer
Which of the following pigments is primarily responsible for capturing light energy during photosynthesis?
Direct answer
Chlorophyll a is the primary pigment involved in photosynthesis, absorbing light most efficiently in the blue and red wavelengths.
Key terms
- Chromatography: A laboratory technique used to separate mixtures into their individual components based on their movement through a stationary phase.
- Rf value: The ratio of the distance traveled by a substance to the distance traveled by the solvent front in chromatography.
Common trap
Misinterpreting Rf values: Remember that a higher Rf value indicates greater solubility and less affinity for the stationary phase.
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Revision notestopic notes
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Open revision notesRelated learning objectives
- Describe the light-dependent reaction including chlorophyll photoionisation, electron transfer, ATP production, reduced NADP and photolysis.
Photosynthesis
- Describe the Calvin cycle using RuBP, GP, triose phosphate, ATP, reduced NADP and rubisco.
Photosynthesis
- Identify environmental factors limiting photosynthesis.
Photosynthesis
- Evaluate data on agricultural practices used to overcome limiting factors.
Photosynthesis
- Required practicals 7 and 8: investigate leaf pigments by chromatography and dehydrogenase activity in chloroplast extracts.
Photosynthesis