Question detail

CGEQ24: Which experimental interpretation best tests Explain transcriptional stimulation and inhibition by transcription factors. using chromatin immunoprecipitation?

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At a glance

MCQ

Type

practice

Style

Topic

Control of gene expression official content

Question

  1. A. CGEQ24: Use chromatin immunoprecipitation. Independent variable: antibody identity. Dependent variable: enriched promoter reads. Controlled variable: sonication time. Method: identify the independent variable, dependent variable and controlled variable; keep controls constant; evaluate validity, reliability, accuracy, precision, uncertainty, risk, limitation, conclusion and improvement. Link the result to Explain transcriptional stimulation and inhibition by transcription factors.; transcription makes RNA and translation makes polypeptide.
  2. B. CGEQ24: Change antibody identity and sonication time together, then ignore uncertainty and controls.
  3. C. CGEQ24: Record enriched promoter reads without a method, dependent variable or evaluation.
  4. D. CGEQ24: Describe gene expression generally but omit the controlled variable and conclusion.

Answer

The correct answer is: CGEQ24: Use chromatin immunoprecipitation. Independent variable: antibody identity. Dependent variable: enriched promoter reads. Controlled variable: sonication time. Method: identify the independent variable, dependent variable and controlled variable; keep controls constant; evaluate validity, reliability, accuracy, precision, uncertainty, risk, limitation, conclusion and improvement. Link the result to Explain transcriptional stimulation and inhibition by transcription factors.; transcription makes RNA and translation makes polypeptide.

Explanation

For this question, start with the A-Level concept in Regulation of transcription and translation, then connect it to the scenario using transcription factor and a clear cause-and-effect chain. CGEQ24: The option is correct because it states method, independent variable, dependent variable, controlled variable, controls, evaluation and a valid conclusion for chromatin immunoprecipitation. Distractors usually fail because they reverse the mechanism, use the wrong scale of organisation, or describe a related topic without answering the exact objective.

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Regulation of transcription and translation common mistake 1

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