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What is the correct sequence of events when inserting a gene into a plasmid using genetic engineering techniques?.
- A.Ligase cuts, gene isolated, plasmid expressed, vector introduced.
- B.Gene isolated, restriction enzymes cut, sticky ends anneal, ligase joins, vector introduced.
- C.Restriction enzymes cut, ligase joins, gene isolated, plasmid expressed.
- D.Vector introduced, gene isolated, sticky ends anneal, restriction enzymes cut.
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