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Cambridge IGCSE Biology practice

Paper 4 structured question: Genetic modification

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Genetic modification

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Paper 4Theory (Extended)2 marks

Genetic modification can be used to produce human proteins in bacteria by inserting a human gene into bacterial plasmids.

Description

Steps in Genetic Modification

The process involves isolating the human gene using restriction enzymes, cutting bacterial plasmid DNA with the same enzymes, joining the human gene to the plasmid with DNA ligase, introducing the recombinant plasmid into bacteria, and allowing bacteria to multiply and produce the human protein.

  1. a

    State the role of restriction enzymes in genetic modification.

    Aim for approximately 1 distinct marking point.

    [1]
  2. b

    Identify the enzyme that joins human DNA to plasmid DNA.

    Aim for approximately 1 distinct marking point.

    [1]

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Paper 4 structured question: Genetic modification

Paper 4Theory (Extended)2 marks

Description

Genetic Modification Process

The human gene is isolated and cut with restriction enzymes, bacterial plasmids are cut with the same enzymes, human DNA is inserted into plasmids using DNA ligase, recombinant plasmids are introduced into bacteria, and bacteria multiply and express the human gene.

  1. a

    State why the same restriction enzymes are used to cut both human DNA and bacterial plasmid DNA.

    Aim for approximately 1 distinct marking point.

    [1]
  2. b

    Identify what happens to bacteria after they take up recombinant plasmids.

    Aim for approximately 1 distinct marking point.

    [1]