logo

Cambridge IGCSE Biology revision notes

Osmosis Revision Notes

Cambridge IGCSE revision notes for Osmosis.

At a glance

revision notes

Resource type

Topic

Osmosis

CambridgeIGCSEBiologyMovement into and out of cells

Complete revision guide 1 of 9

Water as a Solvent in Living Organisms

Water dissolves substances to support digestion, excretion, and transport in organisms.

Syllabus source

Section 3, Topic 3.2, page 15, objective 1

Open source document ↗

What you should be able to do

  • ✓Understand why water is an effective solvent in biological systems.
  • ✓Describe how water dissolves substances during digestion, excretion, and transport.
  • ✓Explain the importance of water's solvent properties for enzyme activity and cellular functions.
1

Water's Role as a Universal Solvent

Water molecules are polar, meaning they have positive and negative ends. This polarity allows water to surround and dissolve many different substances, including salts, sugars, gases, and waste products.

Because of this, water is often called a universal solvent in living organisms, enabling essential biological processes to occur efficiently.

  • Water's polarity allows it to dissolve a wide range of substances.
  • It forms hydrogen bonds with other polar molecules, aiding dissolution.
2

Water in Digestion

During digestion, water dissolves food molecules, breaking them into smaller particles that enzymes can act upon more effectively.

Dissolving food increases the surface area exposed to enzymes, speeding up chemical breakdown and nutrient absorption.

  • Water dissolves food molecules to facilitate enzyme action.
  • Enzymes only work on dissolved substances.
3

Water in Excretion and Transport

Water dissolves waste products such as urea, allowing them to be transported in urine and removed from the body.

In animals, water carries dissolved nutrients, oxygen, and carbon dioxide in the blood to and from cells.

In plants, water transports dissolved minerals and sugars through the sap in xylem and phloem vessels.

  • Water dissolves wastes for excretion.
  • Water transports dissolved substances in blood and plant sap.
  • This transport supports cellular metabolism and homeostasis.

At-a-glance summary

Comparison table

ProcessRole of Water as Solvent
DigestionDissolves food molecules so enzymes can break them down
ExcretionDissolves waste products for removal from the body
TransportCarries dissolved nutrients, gases, and wastes in blood or sap
Enzyme ActivityEnzymes act only on dissolved substrates, so water enables reactions

Active recall

Quick check

1. Why is water called a universal solvent in living organisms?

Because its polarity allows it to dissolve many different substances needed for biological processes.

2. How does water aid digestion?

By dissolving food molecules so enzymes can break them down efficiently.

3. What role does water play in excretion?

It dissolves waste products so they can be removed from the body.

Complete revision guide 2 of 9

Water Movement Through Partially Permeable Membranes by Osmosis

Water moves through partially permeable membranes from higher to lower water concentration by osmosis.

Syllabus source

Section 3, Topic 3.2, page 15, objective 2

Open source document ↗

What you should be able to do

  • ✓Define osmosis and identify the type of membrane involved.
  • ✓State the direction of water movement during osmosis.
  • ✓Understand that osmosis is a passive process requiring no energy.
1

What is Osmosis?

Osmosis is the movement of water molecules through a partially permeable membrane from a region of higher water concentration to a region of lower water concentration.

A partially permeable membrane allows water molecules to pass but blocks many solutes, enabling selective movement of water.

Osmosis occurs to balance water concentrations on both sides of the membrane.

  • Water moves only, solutes do not cross during osmosis.
  • Membrane is selectively permeable to water.
  • Movement is passive, requiring no energy.
2

Direction and Importance of Osmosis

Water moves down its concentration gradient, from where water molecules are more abundant to where they are less abundant.

This process helps cells maintain their water balance, preventing them from swelling or shrinking excessively.

Osmosis is essential for many biological functions, including nutrient absorption and waste removal.

  • Water moves from high to low water concentration.
  • Osmosis maintains cell volume and function.
  • It is a passive process driven by concentration differences.
3

Membrane Characteristics

Partially permeable membranes allow water molecules to pass freely but restrict larger molecules or ions.

This selective permeability is crucial for controlling water movement and maintaining cellular homeostasis.

  • Membrane blocks many solutes but allows water.
  • Selective permeability enables osmosis.
  • Membrane controls internal environment of cells.

At-a-glance summary

Comparison table

FeatureDescription
Membrane TypePartially permeable membrane allowing water but not many solutes
Water MovementFrom higher to lower water concentration
Energy RequirementPassive process, no energy needed
Solute MovementSolutes generally do not move during osmosis

Active recall

Quick check

1. What type of membrane is involved in osmosis?

A partially permeable membrane.

2. In which direction does water move during osmosis?

From an area of higher water concentration to an area of lower water concentration.

3. Does osmosis require energy?

No, osmosis is a passive process and does not require energy.

Complete revision guide 3 of 9

Water Movement Into and Out of Cells by Osmosis

Water enters and exits cells through the cell membrane by osmosis, maintaining cell water balance.

Syllabus source

Section 3, Topic 3.2, page 15, objective 3

Open source document ↗

What you should be able to do

  • ✓State that water moves through the cell membrane by osmosis.
  • ✓Describe the direction of water movement depending on concentration differences.
  • ✓Understand the effects of water movement on cell volume and function.
1

Osmosis Across the Cell Membrane

The cell membrane is selectively permeable, allowing water molecules to pass but restricting many solutes.

Water moves through the membrane by osmosis, from areas of higher water concentration (less solute) to lower water concentration (more solute).

This movement is passive and does not require energy.

  • Cell membrane controls water movement.
  • Water moves down its concentration gradient by osmosis.
  • Osmosis is passive and energy-free.
2

Effects of Water Movement on Cells

If the fluid outside the cell has a higher water concentration than inside, water enters the cell, causing it to swell.

If the outside fluid has a lower water concentration, water leaves the cell, causing it to shrink.

Maintaining proper water balance is vital for cell survival and function.

  • Water entering cells causes swelling.
  • Water leaving cells causes shrinking.
  • Osmosis helps maintain cell volume and health.
3

Importance of Osmosis in Cells

Osmosis regulates water content, preventing cells from bursting or becoming dehydrated.

It supports nutrient uptake and waste removal by controlling water flow.

Understanding osmosis explains how cells interact with their environment.

  • Osmosis maintains cell water balance.
  • It supports essential cellular processes.
  • Cell membrane selectively regulates water movement.

At-a-glance summary

Comparison table

ConditionWater MovementEffect on Cell
Higher water concentration outsideWater enters cellCell swells, may burst if excessive
Equal water concentrationNo net water movementCell volume remains stable
Lower water concentration outsideWater leaves cellCell shrinks and may become dehydrated
Cell membraneSelectively permeableAllows water but restricts many solutes

Active recall

Quick check

1. Through which cell structure does water enter and exit?

The cell membrane.

2. In osmosis, water moves from an area of ___ water concentration to an area of ___ water concentration.

Higher; lower.

3. What happens if too much water leaves a cell?

The cell shrinks and may become dehydrated.

Complete revision guide 4 of 9

Investigating Osmosis Using Dialysis Tubing

This note explains how to investigate osmosis by using dialysis tubing as a model for a selectively permeable membrane. It covers the role of dialysis tubing, identification and control of variables, measurement techniques, safety precautions, and practical tips to ensure reliable results.

Syllabus source

Section 3, Topic 3.2, page 15, objective 4

Open source document ↗

What you should be able to do

  • ✓Understand how dialysis tubing simulates a selectively permeable membrane in osmosis investigations.
  • ✓Identify independent, dependent, and control variables in the experiment.
  • ✓Describe accurate measurement methods and the importance of repeating the experiment.
  • ✓Recognize necessary safety precautions when conducting the investigation.
1

Using Dialysis Tubing to Model Osmosis

Dialysis tubing acts as an artificial selectively permeable membrane, allowing water molecules to pass through while blocking larger solute molecules such as sugar.

When the tubing is filled with a sugar solution and placed in distilled water, water moves by osmosis from the area of lower solute concentration (outside) to higher solute concentration (inside).

This movement causes the tubing to swell and increases its mass or volume, demonstrating osmosis in action.

  • Dialysis tubing mimics a cell membrane's selective permeability.
  • Water moves into tubing if internal sugar concentration is higher.
  • Osmosis causes swelling and mass increase of the tubing.
2

Variables and Measurement in the Investigation

The independent variable is the sugar concentration inside the dialysis tubing, which is deliberately changed to observe its effect.

The dependent variable is the change in mass or volume of the dialysis tubing after immersion in distilled water, reflecting water movement.

Control variables such as temperature, duration of the experiment, and volume of solutions must be kept constant to ensure a fair test.

Accurate measurement of mass before and after the experiment using a balance is essential to quantify osmosis.

Repeating the experiment multiple times improves reliability by reducing random errors.

  • Independent variable: sugar concentration inside tubing.
  • Dependent variable: change in mass or volume of tubing.
  • Control variables: temperature, time, solution volume.
  • Use precise balances for measurement.
  • Repeat trials to confirm results.
3

Safety and Practical Tips for Reliable Results

Handle glassware and solutions carefully to avoid spills and injury during the experiment.

Ensure the dialysis tubing ends are securely tied to prevent leaks of the sugar solution.

Blot the tubing dry before weighing to avoid excess water affecting mass measurements.

Conduct the experiment at room temperature to maintain consistent conditions and control variables.

Record all measurements carefully and maintain consistent timing for each trial.

  • Handle equipment carefully to prevent accidents.
  • Secure tubing ends to avoid leaks.
  • Dry tubing before weighing for accuracy.
  • Maintain consistent temperature and timing.

At-a-glance summary

Comparison table

Variable TypeExample in Osmosis Investigation
Independent VariableSugar concentration inside dialysis tubing
Dependent VariableChange in mass or volume of dialysis tubing
Control VariablesTemperature, duration, volume of solutions
Measurement MethodWeigh tubing before and after experiment

Active recall

Quick check

1. What role does dialysis tubing play in an osmosis investigation?

It acts as a selectively permeable membrane allowing water to pass but not larger solutes like sugar.

2. What is the independent variable in this osmosis experiment?

The sugar concentration inside the dialysis tubing.

3. How is the dependent variable measured in this investigation?

By weighing the dialysis tubing before and after immersion and calculating the change in mass.

Complete revision guide 5 of 9

Investigating Osmosis in Plant Tissues Using Different Solution Concentrations

Immersing plant tissues in solutions of varying concentrations causes water movement by osmosis, changing tissue mass.

Syllabus source

Section 3, Topic 3.2, page 15, objective 5

Open source document ↗

What you should be able to do

  • ✓Describe how solution concentration affects water movement in plant tissues.
  • ✓Identify independent, dependent, and control variables in the investigation.
  • ✓Explain how to calculate percentage change in mass.
  • ✓Understand safety precautions when handling plant tissues and equipment.
  • ✓Recognize the importance of repeating experiments for reliability.
1

Osmosis in Plant Tissues

When plant tissues like potato cylinders are placed in solutions with different solute concentrations, water moves across their cell membranes by osmosis.

If the external solution is hypertonic (more concentrated than cell sap), water leaves the cells, causing the tissue to lose mass and become flaccid.

If the solution is hypotonic (less concentrated), water enters the cells, increasing mass and causing swelling.

In isotonic solutions, solute concentrations are equal inside and outside, so there is no net water movement and mass remains stable.

  • Water moves by osmosis depending on solution concentration.
  • Hypertonic solution causes water loss and mass decrease.
  • Hypotonic solution causes water gain and mass increase.
  • Isotonic solution causes no net water movement.
2

Variables and Measurements

The independent variable is the concentration of the solution in which the plant tissue is immersed.

The dependent variable is the change in mass of the plant tissue after immersion.

Control variables include size and type of tissue, temperature, and immersion time to ensure fair testing.

Percentage change in mass is calculated using: ((Final mass - Initial mass) ÷ Initial mass) × 100.

Repeating the experiment improves reliability and identifies anomalies.

  • Independent variable: solution concentration.
  • Dependent variable: tissue mass change.
  • Control variables: tissue size, temperature, time.
  • Calculate percentage mass change.
  • Repeat for reliable results.
3

Safety and Practical Tips

Use a sharp knife carefully, cutting away from yourself to avoid injury.

Handle solutions and equipment carefully to prevent spills and accidents.

Blot plant tissues dry before weighing to avoid excess water affecting mass measurements.

Maintain consistent temperature and timing during the experiment.

  • Handle sharp tools with care.
  • Avoid spills and contamination.
  • Dry tissues before weighing.
  • Keep conditions consistent.

At-a-glance summary

Comparison table

Solution TypeWater MovementEffect on Plant Tissue Mass
Hypotonic (less concentrated)Water enters cellsMass increases; tissue swells
Isotonic (equal concentration)No net water movementMass remains stable
Hypertonic (more concentrated)Water leaves cellsMass decreases; tissue becomes flaccid
Control VariablesTissue size, temperature, immersion timeEnsure fair test conditions

Active recall

Quick check

1. What is the independent variable in this osmosis investigation?

The concentration of the solution in which the plant tissue is placed.

2. How do you calculate percentage change in mass?

((Final mass - Initial mass) ÷ Initial mass) × 100.

3. What happens to plant tissue in a hypertonic solution?

Water leaves the cells, causing the tissue to lose mass and become flaccid.

Complete revision guide 6 of 9

How Water Pressure Supports Plant Structure

Plants maintain their upright shape because water inside their cells pushes against the cell walls, creating support.

Syllabus source

Section 3, Topic 3.2, page 15, objective 6

Open source document ↗

What you should be able to do

  • ✓Understand the role of water inside plant cells in providing support.
  • ✓Recognize the term turgor pressure and its effect on plant firmness.
  • ✓Explain why plants wilt when they lose water.
1

Turgor Pressure in Plant Cells

Plant cells contain water within their central vacuole and cytoplasm. This water creates an internal pressure called turgor pressure, which pushes the cell membrane against the rigid cell wall.

The cell wall is strong and does not stretch easily, so the pressure from inside keeps the cell firm and helps the plant maintain its shape.

  • Water inside cells creates turgor pressure.
  • Turgor pressure presses the cell membrane against the cell wall.
  • This pressure supports the plant's structure and keeps it upright.
2

Effect of Water Loss on Plant Support

When a plant loses water, the amount of water inside its cells decreases, reducing turgor pressure.

Lower turgor pressure means the cells become less firm, causing the plant to wilt and lose its upright shape.

  • Loss of water reduces turgor pressure.
  • Reduced pressure causes cells to become flaccid.
  • Plants wilt when turgor pressure drops.
3

Importance of Water for Plant Health

Maintaining adequate water levels inside cells is essential for plants to stay firm and healthy.

Watering plants helps maintain turgor pressure, preventing wilting and supporting growth.

  • Water is essential to maintain turgor pressure.
  • Turgor pressure keeps plants firm and upright.
  • Proper watering prevents wilting.

At-a-glance summary

Comparison table

ConditionWater MovementCell PressureCell AppearancePlant Appearance
Adequate waterWater fills cellsHigh turgor pressureCells are firm (turgid)Plant is upright and healthy
Water lossWater leaves cellsLow turgor pressureCells become flaccidPlant wilts and droops
No waterCells lose most waterNo turgor pressureCells collapsePlant is wilted and may die
Air or dryWater evaporatesTurgor pressure dropsCells shrinkPlant wilts

Active recall

Quick check

1. What creates the pressure inside plant cells that supports the plant?

Water inside the cells creates pressure against the cell wall, called turgor pressure.

2. What happens to plant cells when they lose water?

They lose turgor pressure, become flaccid, and the plant wilts.

3. Why does turgor pressure keep plants upright?

Because it pushes the cell membrane against the rigid cell wall, making cells firm and supporting the plant structure.

Complete revision guide 7 of 9

Osmosis, water potential and membranes

Build the complete definition of osmosis and apply its direction to two regions separated by a partially permeable membrane.

Syllabus source

Section 3, Topic 3.2, page 15–16, objective 7

Open source document ↗

What you should be able to do

  • ✓State the complete definition of osmosis.
  • ✓Determine the direction of net water movement from water-potential information.
  • ✓Explain the roles of net movement and a partially permeable membrane.
1

The complete definition

Osmosis is the net movement of water molecules from a region of higher water potential to a region of lower water potential through a partially permeable membrane.

Each part of the definition can earn credit, so include the particles, direction and membrane.

  • Particle: water molecules.
  • Overall direction: higher water potential to lower water potential.
  • Barrier: a partially permeable membrane.
  • Movement: net movement.
2

What net movement means

Water molecules move randomly in both directions through the membrane. Net movement describes the overall result: more water molecules move in one direction than in the other.

The overall direction is towards the region with the lower water potential.

3

Role of the membrane

A partially permeable membrane allows some small molecules, including water, to pass through but prevents some other particles from crossing.

Without the membrane condition, the process described is not a complete definition of osmosis.

4

A reliable reasoning method

When two regions are labelled, compare their water potentials before writing the direction.

  • Step 1: identify the region with the higher water potential.
  • Step 2: identify the region with the lower water potential.
  • Step 3: draw or state the direction from higher to lower water potential.
  • Step 4: mention that movement is through a partially permeable membrane.

At-a-glance summary

Comparison table

Definition componentRequired wording
What moves?Water molecules
What kind of movement?Net movement
From where?Higher water potential
To where?Lower water potential
Through what?A partially permeable membrane

Active recall

Quick check

1. What particles move during osmosis?

Water molecules.

2. Region A has a higher water potential than region B. What is the net direction of water movement?

From region A to region B, through the partially permeable membrane.

3. Why is the word net important?

Water moves in both directions, but more water molecules move in the stated direction overall.

Complete revision guide 8 of 9

Plant Cell Responses to Different Solution Concentrations

Plant cells change their firmness depending on the concentration of the surrounding solution due to osmosis, affecting turgor pressure and plasmolysis.

Syllabus source

Section 3, Topic 3.2, page 15, objective 8

Open source document ↗

What you should be able to do

  • ✓Explain how water moves into or out of plant cells in different solutions.
  • ✓Understand terms: turgid, flaccid, plasmolysis, and turgor pressure.
  • ✓Describe the effects of dilute, concentrated, and isotonic solutions on plant cells.
1

Water Movement and Turgor Pressure

When a plant cell is placed in a dilute (hypotonic) solution, water enters the cell by osmosis because the solution has a higher water potential than the cell sap.

Water entering the cell fills the central vacuole, increasing turgor pressure, which pushes the cytoplasm against the cell wall, making the cell turgid and firm.

  • Water moves into the cell by osmosis in dilute solutions.
  • Turgor pressure increases, making cells firm (turgid).
  • Turgid cells support the plant structure.
2

Effects of Concentrated Solutions and Plasmolysis

In a concentrated (hypertonic) solution, water moves out of the plant cell by osmosis because the external solution has lower water potential.

Loss of water causes the vacuole and cytoplasm to shrink, and the cell membrane pulls away from the cell wall, a process called plasmolysis.

The cell becomes flaccid and limp, reducing support and causing the plant to wilt.

  • Water leaves the cell in concentrated solutions.
  • Plasmolysis is the shrinking of the cell membrane away from the cell wall.
  • Cells become flaccid and plants wilt.
3

Isotonic Solutions and Cell Stability

In an isotonic solution, the concentration of solutes inside and outside the cell is equal.

There is no net movement of water, so the cell remains in its normal state, neither turgid nor plasmolysed.

  • No net water movement in isotonic solutions.
  • Cells maintain normal turgor pressure.
  • Plant cells remain stable in isotonic conditions.

At-a-glance summary

Comparison table

Solution TypeWater MovementCell ConditionTurgor PressurePlant Appearance
Dilute (Hypotonic)Water enters cellTurgidHighFirm and upright
IsotonicNo net water movementNormalStableNormal
Concentrated (Hypertonic)Water leaves cellPlasmolysedLowWilted and limp
Very concentratedSevere water lossFlaccidVery lowSeverely wilted or dead

Active recall

Quick check

1. What happens to a plant cell in a dilute solution?

Water enters by osmosis, increasing turgor pressure and making the cell turgid.

2. Define plasmolysis.

Plasmolysis is when the cell membrane pulls away from the cell wall due to water loss in a concentrated solution.

3. What is the condition of a plant cell in an isotonic solution?

There is no net water movement; the cell remains normal with stable turgor pressure.

Complete revision guide 9 of 9

Water Potential and Osmosis in Organisms

Water potential and osmosis explain how organisms absorb and lose water to maintain cell function and water balance.

Syllabus source

Section 3, Topic 3.2, page 15, objective 9

Open source document ↗

What you should be able to do

  • ✓Understand the concept of water potential and its role in water movement.
  • ✓Explain osmosis as water movement across a partially permeable membrane.
  • ✓Describe how organisms use osmosis to absorb or lose water depending on water potential differences.
1

Water Potential and Direction of Water Movement

Water potential measures the potential energy of water in a system and determines the direction water moves.

Water moves from areas of higher water potential to areas of lower water potential.

This movement balances solute concentrations and water distribution.

  • Water potential indicates water's tendency to move.
  • Water flows from higher to lower water potential.
  • Water potential differences drive osmosis.
2

Osmosis and Cell Membranes

Osmosis is the diffusion of water across a partially permeable membrane from a dilute solution (higher water potential) to a concentrated solution (lower water potential).

Partially permeable membranes allow water to pass but block many solutes.

Osmosis helps cells regulate their water content and maintain turgidity.

  • Osmosis moves water across membranes.
  • Water moves from dilute to concentrated solutions.
  • Membranes are selectively permeable.
3

Significance of Osmosis in Organisms

Cells absorb water when the external environment has higher water potential, keeping cells turgid and functional.

Cells lose water when the environment has lower water potential, which can cause plasmolysis or shrinkage.

Maintaining water balance through osmosis is vital for nutrient transport, cell shape, and survival.

  • Osmosis controls water absorption and loss.
  • Water balance is essential for cell health.
  • Organisms adapt to regulate water potential and osmosis.

At-a-glance summary

Comparison table

ScenarioWater Potential Outside CellWater Potential Inside CellWater MovementEffect on Cell
External solution has higher water potentialHigherLowerWater enters cellCell swells and becomes turgid
External solution has lower water potentialLowerHigherWater leaves cellCell shrinks and may plasmolyse
External solution has equal water potentialEqualEqualNo net movementCell remains stable
Animal cell in hypotonic solutionHigherLowerWater entersCell may swell or burst
Animal cell in hypertonic solutionLowerHigherWater leavesCell shrinks (crenation)

Active recall

Quick check

1. What is water potential?

Water potential is the measure of the potential energy of water, indicating the direction water will move.

2. Define osmosis.

Osmosis is the movement of water across a partially permeable membrane from higher to lower water potential.

3. Why is osmosis important for cells?

It helps cells absorb water to stay turgid or lose water to prevent damage, maintaining water balance.

Related topics

Study nearby topics next

Revision hubs

Keep studying from the right hub